Part 1. General Microbiology, Immunology

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Medical Microbiology, Virology and Immunology. Lecture Notes

Оборот титула

Table of contents

Part 1. General Microbiology, Immunology -

Unit 1. General Microbiology

Lecture 1. Subject and aims of medical microbiology. Morphology and taxonomy of microorganisms. Microscopic research method

Question 1. The subject of medical microbiology and its importance in practical health care. The history of microbiology

Question 2. Taxonomy, system and nomenclature of microorganisms

Question 3. Microbiology research methods

Question 4. Simple and complex staining techniques. The mechanism of smear staining. Staining properties of microorganisms

Question 5. Light microscope, its main characteristics. Types of light microscopy (dark-field, fluorescent). Immersion microscopy principles. Electron microscopy, atomic force microscopy

Question 6. Morphology of bacteria

Question 7. Structure of the bacterial cell: genome, cytoplasm, ribosome. Structure, functions and methods of detection

Question 8. Shell of bacteria: cytoplasmic membrane, cell wall, capsule. Structure, functions and methods of detection

Question 9. Flagella, fimbria (pili). Structure, functions and methods of detection

Question 10. Bacterial spores. Its role and structural features. Spore formation and methods of detection

Lecture 2. Physiology of microorganisms. Metabolism. Nutrition and respiration of bacteria. Cultivation of aerobic and anaerobic bacteria

Question 1. Anabolism and catabolism

Question 2. Mechanisms of nutrients transmission in the bacterial cell

Question 3. Autotrophs and heterotrophs, auxotrophs and prototrophs

Question 4. Classification of culture (nutrient) media

Question 5. Simple and complex nutrient medium

Question 6. Method for isolation of the pure culture

Question 7. Growth phases of bacteria on culture medium

Question 8. Mechanism of bacterial respiration. Aerobes and anaerobes

Question 9. Methods of cultivation of anaerobic bacteria: culture media, equipment

Question 10. The main groups of bacteria enzymes

Question 11. Enzymes in the identification of bacteria. Carbohydrate-splitting enzymes. Proteolytic enzymes

Question 12. Peptolytic enzymes

Question 13. Bacterial enzymes of pathogenicity

Question 14. Isolation of pure culture of aerobes

Question 15. Isolation of pure culture of anaerobes

Lecture 3. Viruses. bacteriophages. Genetics of microorganisms. Biotechnology in microbiology. Molecular-genetic methods of diagnosis

Question 1. Classification of viruses. Virus and virion

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