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Раrt 2. Special Microbiology, Mycology and Virology

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Medical Microbiology, Virology and Immunology. Lecture Notes

Table of contents

Part 1. General Microbiology, Immunology +

Раrt 2. Special Microbiology, Mycology and Virology -

Unit 1. Special Bacteriology and Mycology

Lecture 1. Pathogenic and opportunistic gram-positive and gram-negative cocci

Question 1. Taxonomy and biological properties of Staphylococcus

Question 2. Epidemiology and pathogenesis of diseases caused by Staphylococcus

Question 3. Microbiological diagnosis, treatment and specific prevention of staphylococcal infections

Question 4. Taxonomy and biological properties of Streptococcus

Question 5. Epidemiology and pathogenesis of streptococcal infections

Question 6. The microbiological diagnosis, treatment and specific prevention of streptococcal infections

Question 7. Taxonomy and biological properties of Neisseria meningitidis

Question 8. Epidemiology and pathogenesis of meningococcal infections

Question 9. Microbiological diagnosis, treatment and specific prevention of meningococcal infections

Question 10. Taxonomy and biological properties of Neisseria gonorrhoeae

Question 11. Epidemiology and pathogenesis of gonococcal infections

Question 12. Microbiological diagnosis, treatment and specific prevention of gonococcal infections

Lecture 2. Pathogens of escherichiosis and dysentery. The causative agents of typhoid fever, gastroenteritis and cholera

Question 1. Taxonomy and biological features of Escherichia coli

Question 2. Epidemiology and pathogenesis of diseases caused by E. coli

Question 3. Classification of pathogenic E. coli

Question 4. Microbiological diagnosis, treatment and prophylaxis of E. coli-infections

Question 5. Biological features of Salmonella

Question 6. Epidemiology and pathogenesis of typhoid fever

Question 7. Epidemiology and pathogenesis of gastroenteritis

Question 8. Microbiological diagnosis, treatment and prophylaxis of typhoid fever and gastroenteritis

Question 9. Biological features of Shigella

Question 10. Epidemiology and pathogenesis of dysentery

Question 11. Microbiological diagnosis, treatment and prophylaxis of dysentery

Question 12. Biological features of Vibrio cholerae

Question 13. Epidemiology and pathogenesis of cholera

Question 14. Pathogenesis of cholera

Question 15. Microbiological diagnosis, treatment and prophylaxis of cholera

Lecture 3. Pathogens of diphtheria, whooping cough. Pathogens of legionellosis. Pathogenic mycobacteria

Question 1. Biological features of Corynebacterium diphteriae

Question 2. Epidemiology and pathogenesis of diphtheria

Question 3. Microbiological diagnosis, treatment and prophylaxis of diphtheria

Question 4. Biological features of Bordetella pertussis

Question 5. Epidemiology and pathogenesis of whooping cough

Question 6. Microbiological diagnosis, treatment and prophylaxis of whooping cough

Question 7. Biological features of Mycobacterium

Question 8. Epidemiology and pathogenesis of tuberculosis

Question 9. Microbiological diagnosis, treatment and prophylaxis of tuberculosis

Question 10. Biological features of Mycobacterium leprae

Question 11. Epidemiology and pathogenesis of leprosy

Question 12. Microbiological diagnosis, treatment and prophylaxis of leprosy

Question 13. Biological features of Legionella pneumophila

Question 14. Epidemiology and pathogenesis of Legionella-infections

Question 15. Microbiological diagnosis, treatment and prophylaxis of Legionella-infections

Lecture 4. Pathogens of zoonotic infections: plague and anthrax. Pathogens of brucellosis, tularemia

Question 1. Biological properties of Yersinia pestis

Question 2. Epidemiology and pathogenesis of plague

Question 3. Microbiological diagnosis, treatment and prophylaxis of plague

Question 4. Biological properties of Francisella tularensis

Question 5. Epidemiology and pathogenesis of tularemia

Question 6. Microbiological diagnosis, treatment and prophylaxis of tularemia

Question 7. Biological properties of Brucella

Question 8. Epidemiology and pathogenesis of brucellosis

Question 9. Microbiological diagnosis, treatment and prophylaxis of brucellosis

Question 10. Biological properties of Bacillus anthracis

Question 2. Role, value, and tasks of sanitary microbiology. Indicator microorganisms

Question 3. Microflora of water. Methods of microbiological research of water. Indicator microorganisms of water

Question 4. Microflora of soil. Methods of microbiological research of soil. Indicator microorganisms of soil

Question 5. Microflora of air. Methods of microbiological research of air. Indicator microorganisms of air

Question 6. The normal microflora of the human body and its significance

Question 7. Factors that change the normal microflora of the organism. Dysbacteriosis, ways of its treatment

Test for unit 1

Unit 2. Immunology

Lecture 1. Immunology as a science. Introduction in immunology

Question 1. Immunology, its definition

Question 2. The history of immunology, the main periods in the development of immunology

Question 3. Sections of modern immunology. The concept of immunity. Classification of immunity

Question 4. Structure of the immune system. The primary (central) organs of the immune system

Question 5. The secondary organs of the immune system

Question 6. The role of blood cells in immunity

Question 7. The primary cell populations of the immune system

Question 8. Scheme of the immune response

Lecture 2. Innate immunity. Factors and mechanisms of the innate immunity. The concept of a non-specific (innate) immunity. The main barriers of nonspecific immunity

Question 1. The concept of a non-specific (innate) immunity. The main barriers of nonspecific immunity

Question 2. The innate immune factors — skin and mucous membranes

Question 3. Lysozyme — chemical composition and function. Normal microflora in human immunity

Question 4. Complement — chemical composition and functions

Question 5. The activation of the complement system

Question 6. Phagocytosis, mononuclear phagocyte system

Question 7. The functions of phagocytes. Opsonins

Question 8. Phagocytosis mechanism stage. «Oxygen explosion». Complete and incomplete phagocytosis

Question 9. Cytokines — definition, properties. The therapeutic use of cytokines

Question 10. Classification of cytokines

Question 11. Cytokines: interleukins, interferons

Question 12. Cytokines: tumour necrosis factor, colony-stimulating factors, transforming growth factor-β

Question 13. Protective serum proteins

Lecture 3. Antigens. Antibodies

Question 1. Antigens: definition, chemical composition

Question 2. The structure of the antigen

Question 3. Properties of antigens: antigenicity, immunogenicity, specificity, foreignness

Question 4. Classification antigens on its origin, the degree of immunogenicity

Question 5. Classification of antigens by the immune response. Haptens. Adjuvants

Question 6. The antigens of the human body

Question 7. The antigens of bacteria, viruses. autoantigens

Question 8. Antibodies: definition, structure

Question 9. Immunoglobulin classes. serum immunoglobulins

Question 10. The properties of antibodies

Question 11. Phases of antibody production

Question 12. Protective functions of antibodies

Lecture 4. Hypersensitivity. Immunological memory. Immunological tolerance

Question 1. Hypersensitivity, its definition. The definition of allergy. Stages of an allergic reaction

Question 2. Classification of allergies

Question 3. Type I of immediate-type hypersensitivity: anaphylaxis (mechanism of development, manifestations of anaphylaxis, its treatment). Anaphylactic shock

Question 4. Type II of immediate hypersensitivity (cytotoxic): mechanisms of development, clinical manifestations

Question 5. III type of immediate hypersensitivity reactions (immune complex), mechanisms of development, clinical manifestations

Question 6. Delayed type of hypersensitivity: mechanism of development, manifestations of contact allergy

Question 7. Classification of allergens. infectious allergy

Question 8. Drug allergies: the immune response to the drug (haptens), clinical manifestations of drug allergy

Question 9. Laboratory diagnosis of allergies

Question 10. Immunological memory: concept, mechanisms

Question 11. Immunological tolerance: concept, causes, mechanisms

Lecture 5. Pathology of the immune system

Question 1. The definition of immunodeficiency, the classification of immunodeficiencies

Question 2. Primary immunodeficiencies: characteristics, classification

Question 3. Primary immunodeficiencies: T-cell deficiencies

Question 4. Primary immunodeficiencies: combined B-cell and T-cell deficiencies

Question 5. Primary immunodeficiencies: disorders of the complement system (complement deficiencies)

Question 6. Primary immunodeficiencies: phagocyte deficiencies

Question 7. Secondary (acquired) immunodeficiency: causes, characteristics, classification

Question 8. Clinical manifestations of immunodeficiency states

Lecture 6. Evaluation of the immune status

Question 1. Laboratory diagnosis of immunodeficiencies in children and adults: methods, assessment criteria, interpretation

Question 2. Methods of assessing the immune status

Question 3. The levels of evaluation of the immune status

Question 4. First-stage tests (tentative)

Question 5. The second-stage tests (analytical) in the evaluation of the immune status

Question 6. General rules of immunogram evaluation

Lecture 7. Serological tests: agglutination, precipitation test, complement fixation test, tests using labeled antibodies and antigens (ELISA, IFT, RIA)

Question 1. Serological tests: definition, classification and practical use

Question 2. Agglutination test: definition, mechanism, classification and practical use

Question 3. Direct agglutination tests: mechanism and practical use

Question 4. Indirect (passive) agglutination tests: mechanism and practical use

Question 5. Precipitation reaction: identification, mechanism, types, practical use

Question 6. Complement fixation test (CFT): the purpose of its carrying out, components, mechanisms

Question 7. Labelling methods for detection antigen-antibody binding

Question 8. Immunofluorescent test

Question 9. Enzyme-linked immunosorbent assay

Question 10. Radioimmunoassay (RIA)

Question 11. Western blotting (immunoblotting)

Lecture 8. Specific prevention and treatment of infectious diseases. Therapeutic, prophylactic, and diagnostic immunological preparations

Question 1. Immunoprophylaxis, immunotherapy: definitions

Question 2. The history of immunisation

Question 3. Vaccines — classification, characteristics

Question 4. Complications after vaccines, contraindications to immunisation. scheme of vaccine use

Question 5. The immunological antibody-based drugs. Classification. Characteristic (passive immunisation)

Question 6. Rules of vaccines and serums injection. Routes of vaccine injection

Lecture 9. Antiviral and antibacterial immunity. immunity in transplantation, cancer and immunity in mycoses. Immunity and age

Question 1. Features of local immunity — skin and mucous membranes

Question 2. Antibacterial and antitoxic immunity. Factors of antibacterial immunity

Question 3. Antiviral immunity

Question 4. Antifungal immunity

Question 5. Immunity in protozoa and parasitic infections

Question 6. Immunity in transplantation

Question 7. Antitumor immunity

Question 8. Immunity in pregnancy

Question 9. Age features of immunity

Test for unit 2

Раrt 2. Special Microbiology, Mycology and Virology +

Key answers for Test

List of literature